Table 2 The total yeast counts and frequency of yeast species on WLN agar. F1 and F2, inoculated with pure cultures of RC212 (LALVIN) and R312 (isolated from Xinjiang, China, and made into active dry yeasts in our previous study) after crushing (t = 0); F3, inoculated with both RC212 and R312 at the same time (t = 0); F4, where RC212 was inoculated 48 h (t = 48) later than R312 (t = 0); F5, where RC212 was inoculated 48 h (t = 0) earlier than R312 (t = 48)
Yeast | Number of yeast cells ( × 107 CFU ml−1) a /Colony frequency (%) b | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Stage 0 | F1 | F2 | F3 | F4 | F5 | |||||||||||
Stage 1 | Stage 2 | Stage 3 | Stage 1 | Stage 2 | Stage 3 | Stage 1 | Stage 2 | Stage3 | Stage 1 | Stage 2 | Stage 3 | Stage 1 | Stage 2 | Stage 3 | ||
S. cerevisiae | — | 0.95 ± 4.60/29.23 | 75 ± 4.43/68.18 | 18.5 ± 9.36/92.50 | 4.3 ± 7.27/65.15 | 180 ± 8.39/53.73 | 19.5 ± 14.53/100 | 220 ± 5.44/74.58 | 11.5 ± 7.77/74.19 | 13 ± 7.72/100 | 23 ± 16.17/88.46 | 16.5 ± 11.17/78.57 | 325 ± 13.84.02/98.48 | 0.45 ± 11.15/29.03 | 90 ± 12.35/54.55 | 455 ± 10.37/98.91 |
Hanseniaspora sp. | 0.004 ± 4.58/20 | 2 ± 8.53/61.54 | 35 ± 4.86/31.82 | 1.5 ± 6.78/7.50 | 1.7 ± 5.80/25.76 | 145 ± 9.53/43.28 | — | 55 ± 11.61/18.64 | 3 ± 9.84/19.35 | — | 2 ± 12.10/7.69 | 4 ± 6.93/19.05 | 5 ± 4.34/1.52 | 0.9 ± 16.42/58.06 | 60 ± 11.24/36.36 | 5 ± 0.84/1.09 |
Issatchenkia sp. | 0.0135 ± 5.57/67.5 | 0.3 ± 4.29/9.23 | — | — | 0.6 ± 2.53/9.09 | 10 ± 5.59/2.99 | — | 20 ± 8.25/6.78 | 0.5 ± 2.28/3.23 | — | 0.5 ± 3.52/1.92 | 0.5 ± 5.06/2.38 | — | 0.1 ± 6.81/6.45 | 15 ± 4.56/9.09 | — |
C. diversa | 0.0025 ± 7.21/12.5 | — | — | — | — | — | — | — | 0.5 ± 4.15/3.23 | — | 0.5 ± 4.98/1.92 | — | — | 0.1 ± 3.85/6.45 | — | — |
Total c | 0.02 ± 7.55 | 3.25 ± 14.24 | 110 ± 8.02 | 20 ± 11.68 | 6.6 ± 9.12 | 335 ± 15.07 | 19.5 ± 14.53 | 295 ± 8.97 | 15.5 ± 8.99 | 13 ± 7.72 | 26 ± 15.03 | 21 ± 14.46 | 330 ± 11.64 | 1.55 ± 11.21 | 165 ± 17.44 | 460 ± 11.08 |