Table 2 A Summary of treatments used for the purification of β-xylosidase from A. tamarii
Treatment | Total protein (mg) | Total activity (U) | Specific activity (U/mg protein) | Recovery (%) | Purification (folds) |
|---|---|---|---|---|---|
Cell-free filtrate (200 ml) | 2,034 | 8,200 | 4.03 | 100 | 1.0 |
Cell-free dialysate | 2,033 | 8,112 | 3.99 | 98.92 | 0.99 |
Cell-free precipitation (NH4)2SO4 (60 %) | 481 | 5,927 | 12.32 | 72.28 | 3.05 |
Gel filtration (Sephadex G-100) | 98.6 | 4,435 | 44.97 | 54.08 | 11.15 |
Ion-exchange chromatography DEAE-cellulose | 23.1 | 3,327 | 144.02 | 40.57 | 35.73 |