Phenotypic and genotypic antibiotic resistance profiles of Escherichia coli O157 from cattle and slaughterhouse wastewater isolates

Ayaz, Naim Deniz; Gencay, Yilmaz Emre; Erol, Irfan

doi:10.1007/s13213-014-0961-5

Table 2 Primer pairs, annealing temperatures and PCR protocols used to determine the antibiotic resistance gene profile of E. coli O157:H7⁺/H7^- cattle and wastewater isolates

From: Phenotypic and genotypic antibiotic resistance profiles of Escherichia coli O157 from cattle and slaughterhouse wastewater isolates

Antibiotic	Gene	Annealing (°C)	Nucleotide Sequence (5ʹ - 3ʹ)	Product (bp)	Reference
Ampicillin	ampC	53	TTCTATCAAMACTGGCARCC^a	550	Schwartz et al. (2003)
Ampicillin	ampC	53	CCYTTTTATGTACCCAYGA^a	550	Schwartz et al. (2003)
Tetracycline	tetA	58	GGCCTCAATTTCCTGACG	372	Guillame et al. (2000)
	tetA	58	AAGCAGGATGTAGCCTGTGC	372	Guillame et al. (2000)
	tetB	58	GAGACGCAATCGAATTCGG	228	Guillame et al. (2000)
	tetB	58	TTTAGTGGCTATTCTTCCTGCC	228	Guillame et al. (2000)
	tetC	58	TGCTCAACGGCCTCAACC	379	Guillame et al. (2000)
	tetC	58	AGCAAGACGTAGCCCAGCG	379	Guillame et al. (2000)
	tetD	58	GGATATCTCACCGCATCTGC	436	Guillame et al. (2000)
	tetD	58	CATCCATCCGGAAGTGATAGC	436	Guillame et al. (2000)
	tetE	58	TCCATACGCGAGATGATCTCC	442	Guillame et al. (2000)
	tetE	58	CGATTACAGCTGTCAGGTGGG	442	Guillame et al. (2000)
	tetG	58	CAGCTTTCGGATTCTTACGG	844	Gebreyes and Altier (2002)
	tetG	58	GATTGGTGAGGCTCGTTAGC	844	Gebreyes and Altier (2002)
Florphenicol	floR	55	TATCTCCCTGTCGTTCCAG	399	Keyes et al. (2000)
Florphenicol	floR	55	AGAACTCGCCGATCAATG	399	Keyes et al. (2000)
Chloramphenicol	cmlA	62	CCGCCACGGTGTTGTTGTTATC	698	Gebreyes and Altier (2002)
Chloramphenicol	cmlA	62	CACCTTGCCTGCCCATCATTAG	698	Gebreyes and Altier (2002)
Sulphisoxazole	sulI	58	GTGACGGTGTTCGGCATTCT	779	Lanz et al. (2003)
	sulI	58	TCCGAGAAGGTGATTGCGCT	779	Lanz et al. (2003)
	sulII	58	CGGCATCGTCAACATAACCT	721	Lanz et al. (2003)
	sulII	58	TGTGCGGATGAAGTCAGCTC	721	Lanz et al. (2003)
Streptomycin	strA	56	CTTGGTGATAACGGCAATTC	548	Gebreyes and Altier (2002)
	strA	56	CCAATCGCAGATAGAAGGC	548	Gebreyes and Altier (2002)
	strB	54	ATCGTCAAGGGATTGAAACC	509	Gebreyes and Altier (2002)
	strB	54	GGATCGTAGAACATATTGGC	509	Gebreyes and Altier (2002)
Aminoglycosides	aadA	60	GTGGATGGCGGCCTGAAGCC	525	Lanz et al. (2003)
Aminoglycosides	aadA	60	AATGCCCAGTCGGCAGCG	525	Lanz et al. (2003)

^a According to the IUPAC (International Union of Pure and Applied Chemistry): M, A or C; R, A or G; Y, C or T

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ISSN: 1869-2044

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