Fig. 1

Structural identification of the converted product. a) TLC analysis of the geniposide transformation by Synechocystis sp. PCC 6803. TLC analysis was performed after 25 days of incubation. b) HPLC chromatograms of the biotransformation products of geniposide by Synechocystis sp. PCC 6803. The compounds were eluted with a methanol:water mixture at a ratio of 35:65 (v/v) as the mobile phase at a definite flow rate of 0.8 mL min^-1. The profiles were recorded at 238 nm. All of the groups with the exception of group 1 were incubated in BG-11 liquid medium for 25 days and received the same treatment before HPLC analysis. 1, geniposide standard; 2, geniposide in BG-11 liquid medium in the absence of Synechocystis sp. PCC 6803; 3, geniposide and its converted products transformed by Synechocystis sp. PCC 6803; 4, Synechocystis sp. PCC 6803 in BG-11 liquid medium in the absence of geniposide; A and B, two converted products with different polarities. ①, geniposide; ②, product A; ③, product B