Table 1 The composition of carbon sources used in culture media for Yarrowia lipolytica
Medium | Pseudo components (%)a | ||
|---|---|---|---|
Glucose | Olive oil | Glycerol | |
I experimental scheme (the composition of culture media designed for low levels of the maximum amount of carbon source) | |||
1 | 100 | 0 | 0 |
2 | 0 | 100 | 0 |
3 | 0 | 0 | 100 |
4 | 50 | 50 | 0 |
5 | 50 | 0 | 50 |
6 | 0 | 50 | 50 |
7 | 33.3 | 33.3 | 33.3 |
8 | 66.7 | 16.7 | 16.7 |
9 | 16.7 | 66.7 | 16.7 |
10 | 16.7 | 16.7 | 66.7 |
II experimental scheme (the composition of culture media designed for high levels of the maximum amount of carbon source) | |||
1 (11) | 100 | 0 | 0 |
2 (12) | 0 | 100 | 0 |
3 (13) | 0 | 0 | 100 |
4 (14) | 50 | 50 | 0 |
5 (15) | 50 | 0 | 50 |
6 (16) | 0 | 50 | 50 |
7 (17) | 33.3 | 33.3 | 33.3 |
8 (18) | 66.7 | 16.7 | 16.7 |
9 (19) | 16.7 | 66.7 | 16.7 |
10 (20) | 16.7 | 16.7 | 66.7 |
The composition of culture media designed for validation of a model calculated from experiments according to the I experimental scheme | |||
1w | 0.5 | 0.4 | 0.1 |
2w | 0.1 | 0.72 | 0.18 |
The composition of culture media designed for validation of model calculated from experiments according to the II experimental scheme | |||
3w | 0.5 | 0.4 | 0.1 |
4w | 0.05 | 0.9 | 0.05 |