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Table 4 Biomass yield and lipase activity of Y. lipolytica in YPGO and YPO-Gl30 medium-based bioreactor culture

From: Assortment of carbon sources in medium for Yarrowia lipolytica lipase production: A statistical approach

Mediuma

Carbon source composition

Duration of lag phase (h)

Biomass yield (g d.m./L)

Extracellular lipase activity (U/mL)

Cell-bound lipase activity (U/g d.m.)

YPGO

20 g/L glucose 12.0 mL/L olive oil

14.25 ± 0.25

24.7 ± 1.4

0.140 ± 0.02

10.17 ± 1.93

YPO-Gl30

30 g/L glycerol 19.2 mL/L olive oil

11.25 ± 0.25

18.9 ± 0.4

0.135 ± 0.02

9.19 ± 1.11

  1. d.m., Dry cell mass
  2. The results are presented as the average ± standard deviation (SD)
  3. All culture media, irregardless of the carbon source(s), contained 10.0 g/L yeast extract and 20.0 g/L peptone and had a pH of 5.0. YPGO medium contained 20.0 g/L glucose and 12.0 mL/L olive oil; YPO-Gl30 medium contained 19.2 mL/L olive oil and 30.0 g/L glycerol
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Annals of Microbiology

ISSN: 1869-2044

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