Cloning and characterization of a differentially expressed mitochondrial manganese superoxide dismutase gene from Pleurotus ostreatus

Table 1 Primers for PCR amplification in this study

Primer name	Sequence (5′ → 3′)	Description
MS-R5	AAGGGGTCCTGGTTGGGGGTCGTGGT	Specific primer of PoMn-SOD gene for 5′ RACE PCR
UPM (long)	CTAATACGACTCACTATAGGGCAAGCAGTGGTATC AACGCAGAGT	Universal primer for 5′ RACE PCR
UPM (short)	CTAATACGACTCACTATAGGGC	Universal primer for 5′ RACE PCR
MS-FS	ACTCATTCCCGTTCTACCAT	Primers of PoMn-SOD gene for cDNA and genomic DNA amplification
MS-FA	CGCTGCTAAGTTCATCACAAG
RAPF	GGTTTGAACGCTGCTGAG	Primers of PoMn-SOD gene for quantitative real-time PCR
RAPR	AGTTTGCCTCCGTCACCG	Primers of PoMn-SOD gene for quantitative real-time PCR
α-tubulinF	CCGCTATCTCACCGTCGC	Primers of α-tubulin gene for quantitative real-time PCR
α-tubulinR	GTTCTTGGATGGCGGTCG	Primers of α-tubulin gene for quantitative real-time PCR

ISSN: 1869-2044