Table 3 Synthesis of contributors involved in estimating the value of uncertainty (U) associated with the measure of live and dead cells in samples of active dry yeast. L sample with a low yeast concentration, M sample with a medium yeast concentration, H sample with a high yeast concentration

Repeatability (r)

12 %

12 %

8,7 %

24 %

19 %

26 %

Volume of FCM cuvette

2.9 %

2.9 %

2.9 %

2.9 %

2.9 %

2.9 %

Dilution of ADY sample

2.9 %

2.9 %

2.9 %

2.9 %

2.9 %

2.9 %

Weight of ADY sample

2.9 %

2.9 %

2.9 %

2.9 %

2.9 %

2.9 %

Volume of sample used in the decimal dilutions

0.6 %

0.6 %

0.6 %

0.6 %

0.6 %

0.6 %

Volume of peptone water used in the decimal dilutions

1.0 %

1.0 %

1.0 %

1.0 %

1.0 %

1.0 %

Number of dilutions

Combined relative standard uncertainty (u _c )

13 %

13 %

10 %

25 %

20 %

26 %

Degree of freedom

Coverage factor (k)

Relative expanded uncertainty (U%)

28 %

28 %

21 %

53 %

43 %

57 %

Expanded uncertainty (U)

2.3 × 10⁹

1.2 × 10¹⁰

2.7 × 10¹⁰

9.7 × 10⁸

5.4 × 10⁹

1.7 × 10¹⁰