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Fig. 1 | Annals of Microbiology

Fig. 1

From: Use of biotin-labeled IgY overcomes protein A interference in immunoassays involving Staphylococcus aureus antigens

Fig. 1

Cloning, expression, and purification of the SEB gene of S aureus. a Agarose gel showing the PCR-amplified SEB gene (L1); M- 100 bp DNA ladder. b Coomassie-stained SDS-PAGE gel showing the expression of recombinant SEB protein. M- Prestained protein ladder; Lane 1- Uninduced BL21DE3; Lane 2- Induced BL21DE3; Lane 3- Uninduced BL21DE3 clone carrying a pRSET A-seb plasmid; Lane 4- Induced BL21DE3 clone carrying a pRSET A-seb plasmid showing expressed SEB protein. c Coomassie-stained SDS-PAGE gel showing purified recombinant SEB protein

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Annals of Microbiology

ISSN: 1869-2044

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