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Fig. 4 | Annals of Microbiology

Fig. 4

From: Use of biotin-labeled IgY overcomes protein A interference in immunoassays involving Staphylococcus aureus antigens

Fig. 4

Western blots showing SpA reactivity with normal and biotinylated IgG and IgY antibodies. a Blot treated with normal IgG. Lane M- Prestained protein ladder; Lane 1- FRI 722; Lane 2- NCIM 2122. b Blot treated with biotinylated IgG. Lane M- Prestained protein ladder; Lane 1- FRI 722; Lane 2- NCIM 2122. c Blot treated with normal IgY. Lane M- Prestained protein ladder; Lane 1- ATCC 6538p; Lane 2- FRI 722; Lane 3- NCIM 2122. d Blot treated with biotinylated IgY and streptavidin-HRP. SpA cross-reactivity is not observed with this combination. Lane M- Prestained protein ladder; Lane 1- ATCC 6538p; Lane 2- FRI 722; Lane 3- NCIM 2122. e Blot treated with anti-SpA antibody to test the SpA reactivity. Lane M- prestained protein ladder; lane 1- NCIM 2657; lane 2- FRI 722

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Annals of Microbiology

ISSN: 1869-2044

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