Use of biotin-labeled IgY overcomes protein A interference in immunoassays involving Staphylococcus aureus antigens

Table 2 Comparison of the biotin-IgY ELISA with the biotin-IgG ELISA, western blotting, and PCR on standard and isolated S. aureus strains

Strain	Biotin-IgY ELISA	Biotin-IgG ELISA	Western blot	PCR
Staphylococcus aureus FRI 722	+	+	+	+
S. aureus ATCC 29213	+	+	+	+
S. aureus ATCC 700699	-	+	-	-
S. aureus ATCC 43300	-	+	-	-
S. aureus ATCC 6538p	-	+	-	-
S. aureus NCIM-2122	+	+	+	+
S. aureus NCIM 2654	+	+	+	+
S. aureus NCIM 2127	+	+	+	+
S. aureus NCIM 2122	+	+	+	+
S. aureus NCIM 2120	+	+	+	+
S. aureus NCIM 2657	+	+	+	+
S. aureus NCIM 5021	-	+	-	-
S. aureus NCIM 2794	+	+	+	+
S. aureus NCIM 2492	+	+	+	+
S. aureus NCIM 2079	-	+	-	-
S. aureus IVRI	+	+	+	+
S. aureus E-2215 (Dharwad)	-	+	-	-
S. aureus E-2233 (Dharwad)	-	+	-	-
S. aureus E-1933 (Dharwad)	-	+	-	-
S. aureus E-1903 (Dharwad)	-	+	-	-
S. aureus E-2447 (Dharwad)	-	+	-	-
S. aureus E-2147 (Dharwad)	+	+	+	+
S. aureus E-2459 (Dharwad)	+	+	+	+
S. aureus E-2277 (Dharwad)	-	+	-	-
S. aureus E-2282 (Dharwad)	-	+	-	-
S. aureus E-2393 (Dharwad)	-	+	-	-
S. aureus E-2295 (Dharwad)	-	+	-	-
S. aureus E-1898 (Dharwad)	+	+	+	+

ATCC- American Type Culture Collection, Manassas USA; FRI- Food Research Institute, USA; NCIM- National Collection of Industrial Microorganisms, Pune; Dharwad- Clinical isolates received from SDM medical college, Dharwad, Karnataka, India; IVRI- Indian Veterinary Research Institute, Izzatnagar, Uttar Pradesh, India

ISSN: 1869-2044