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Table 4 Effect of an incubation on ice on the survival of complementing diploids KmR GmR a formed after a Z-mating between MG405 and MG406 (MI) or MG406 and MG409 (MII)

From: Selection and evolution of mating products after spontaneous zygogenesis in Escherichia coli K-12

Exposure to KG

Total CFU mL-1 (Nt)b

S rate (Nt/Noc)

Ratio of S rates M+ vs. M−

Frequency of mixed KmR/GmR CFU among survivors

MI−

MI+

2.8 ± 0.7 × 106

7.9 ± 3.8 × 101

5.5 × 10−1

1.55 × 10−5

2.8 × 10−5

6.9 × 10−2

4.8 × 10−1

MII−

MII+

4.6 ± 1.4 × 107

7.2 ± 5.9 × 102

1.8 × 101

2.8 × 10−4

1.55 × 10−5

2.4 × 10−1

6.9 × 10−1

  1. aCultures exposed to KG for 3 h were subsequently placed on ice for 25 h in the case of MII, 43 h in MI, before centrifugation and plating
  2. bMean of three tubes in MI, four tubes in MII
  3. cNo = 5.1 × 106 CFU mL−1 of minority parent MG405 in MI and 2.6 × 106 CFU ml−1 of MG406 in MII