Table 5 Subclonal analyses of various Z-matings products
From: Selection and evolution of mating products after spontaneous zygogenesis in Escherichia coli K-12
| Â | Cross between | Involved genetic markersa | Selected markers | No analyzed clones subclonesb | No. of isolates of parental recombinant phenotype | Frequency of different phenotypes | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
P1 | P2 | |||||||||||
(Szp+) | (F−) | P1 | P2 | P1/P2 | R1-n | P/ R | ||||||
1 | MG352 | W1S | thr leu lac galK rpsL metB | thr + leu + met + | 5 | 25 (5) | 4 | 2 | 5 | 12 | 2 | 0.20 |
2 | MG352 | JC5519 | thr leu proA lac his argG recBC rpsL metB | thr + leu + his + arg + met + | 4 | 14 (3) | 5 | 5 | 4 | 0 | 0 | 0.20 |
3 | MG352 | MG355 | lac galK argG metB | arg + met + | 6 | 24 (4) | 7 | 10 | 3 | 1 | 3 | 0.25 |
4 | MG392 | GMS343 | lacY galK trpA,B aroD nalA argG rpsL mtl | trp + aro + | 102 | 306 (3) | 75 | 56 | 22 | 61 | 92 | 0.04 |
5 | MG405 | 1485/Nx | thr leu proA lacY trpA,B aphII nalA rpsL | lac + trpA,B + | 9 | 45 (5) | 2 | 6 | 13 | 8 | 16 | 0.18 |
6 | MG405 | KL262 | thr leu proA lacY pyrD trpC his aphII tyrA thyA recA nalA argE | lac + trp + pyr + thy + | 12 | 37 (3) | 18 | 6 | 2 | 6 | 5 | 0.22 |
7 | MG406 | MG409 | thr leu proA lacY gal trpA,B aac2 nalA rpsL argE | lac + trpA,B + | 12 | 38 (3) | 6 | 6 | 3 | 12 | 12 | 0.24 |
8a | MG407 | IL1 | thr leu proA lacY trpC pyrD aroD aphII Aac2 his nalA rpsL argE mtl | lac + pyrD + trp + aro + | 18 | 77 (4) | 9 | 34 | 9 | 17 | 8 | 0.17 |
8b | MG407 | MG405 (IL1 Szp+) | Â | 10 | 40 (4) | 12 | 5 | 8 | 8 | 7 | 0.27 | |