Table 1 Primers used in the PCRs carried out in this work
Primer | Sequence (5′–3′) | Annealing temperature | Amplicon (bp) | Reference |
|---|---|---|---|---|
nuc-F | GCGATTGATGGTGATACGGT | 60 °C | 270 bp | This study |
nuc-R | CAAGCCTTGACGAACTAAAGC | Â | Â | Â |
agr-F | AACTTAGATCATATTCAATTTTTGC | 60 °C | 1889 bp | This study |
agr-R | AGACATTGTCTGCATTATCAGC | Â | Â | Â |
icaA-F | AAACTTGGTGCGGTTACAGG | 54 °C | 188 bp | Reiter et al. 2012 |
icaA-R | GTAGCCAACGTCGACAACTG | Â | Â | Â |
atlA-F | CAGTTAGCAAGATTGCTCAAG | 54 °C | 1035 bp | Reiter et al. 2012 |
atlA-R | CCGTTACCTGTTTCTAATAGG | Â | Â | Â |
sasG-F | ACCACAGGGTGTAGAAGCTAAATC | 54 °C | 188 bp | Reiter et al. 2012 |
sasG-R | CGAGC TTTTCTAA CCTTAGGTGTC | Â | Â | Â |