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Fig. 4 | Annals of Microbiology

Fig. 4

From: In vivo functional expression of an extracellular Ca2+-independent Bacillus pumilus lipase in Bacillus subtilis WB800N

Fig. 4

SDS-PAGE of LipBP in B. subtilis WB800N and recombinant protein purification. a SDS-PAGE of LipBP. Lane M standard marker proteins; lane 1 supernatant of fermentation broth (pHT43); lane 2 supernatant of fermentation broth (WB800N-pHT43-lip BP) after induction for 20 h; lane 3 cell lysate supernatant of WB800N-pHT43-lip BP after induction for 20 h. b Recombinant protein purification. Lane M standard marker proteins; lane 1 purification of LipBP by Ni-NTA affinity chromatography

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Annals of Microbiology

ISSN: 1869-2044

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