Fig. 5
Characterization of recombinant LipBP. a The temperature optimum of LipBP lipase activity (pH 8.0). b The pH optima of LipBP lipase activity (40 °C). c The thermal stability of LipBP lipase activity. The thermal stability of enzyme was evaluated by incubating the enzyme in various temperature for different times (2, 4 and 8 h) and incubated in Tris–HCl buffer (pH 8.0). d The pH stability of LipBP lipase activity. The pH stability of the enzyme was determined by incubating the enzyme in various buffers for different times (2, 4 and 8 h) and incubated at 40 °C. Each value represents the mean ± SD of triplicates