Fig. 6

Effect of metal ions and chemical compounds on the lipase activity. a The concentration of SDS, Tween 20, Tween 80, Tritonx-100, EDTA and DTT was at 1.0 mM. b The metal ions were at two different concentrations (1 and 10 mM). c The organic solvents were at two different concentrations (10 and 25 %) (Met methanol, Eth ethanol, Ace acetonitrile, Ben benzene, Hex n-hexane, Chl chloroform, Acet acetone, Pro n-propanol, Isop isopropanol). The reaction mixtures containing the enzyme sample were incubated at 40 °C for 60 min in Tris–HCl buffer (pH 8.0). The enzyme sample without any additives was considered as control (100 %)