Table 1 The bacterial strains and plasmids used in this study
Strains and plasmids | Relevant features | Source or reference |
|---|---|---|
Strains | ||
E. coli DH5α | Fˉψ80lacZΔM15Δ (lacZYA-argF) U169 endA1 recA1 hsdR17 (rkˉ,mkˉ) supE44λˉthi-1 gyrA96 relA1 phoA | BRL |
B. pumilus Nws-bp1 | wild strain screened from food factory sewage (CCTCC AB 2013050) | CCTCC AB 2013050 |
B. subtilis WB800N WB800N-pHT43-lip BP | apr his npr eglS (DELTA) 102 bglT/bglS (DELTA) EVCmr B. subtilis WB800N, carrying plasmid pHT43-lip BP, Cmr | Mo Bi Tec (Phan et al. 2006) This study |
Plasmids | ||
pMD19-T | Cloning vector; Ampr | Takara |
pMD-lip BP | pMD19-T vector, carrying mature lipase gene, Ampr | This study |
pHT43 | Bacillus-E. coli shuttle vector; vector for extracellular expression; Ampr and Cmr | Mo Bi Tec (Phan et al. 2006) |
pHT43-lip BP | pHT43 based vector, carrying mature lipase gene; Ampr and Cmr | This study |