Table 2 Purification of LipBP by Ni-NTA affinity chromatography
Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification |
|---|---|---|---|---|---|
Crude extract | 1614.5 | 7.6 | 213.3 | 100.0 | 1.0 |
Ni-NTA | 858.9 | 0.3 | 2650 | 53.2 | 12.4 |
Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification |
|---|---|---|---|---|---|
Crude extract | 1614.5 | 7.6 | 213.3 | 100.0 | 1.0 |
Ni-NTA | 858.9 | 0.3 | 2650 | 53.2 | 12.4 |