Table 1 PCR primers for virulence gene detection
Target gene | Primer sequence (5′-3′) | Annealing temperature (°C) | Size (bp) | Reference |
|---|---|---|---|---|
fliC h7 | GCGCTGTCGAGTTCTATCGAGC CAACGGTGACTTTATCGCCATTCC | 59 | 625 | Xu et al. 2008 |
rfbEO157 | GGATGACAAATATCTGCGCTGC GGTGATTCCTTAATTCCTCTCTTTCC | 59 | 291 | |
eaeA | GACCCGGCACAAGCATAAGC CCACCTGCAGCAACAAGAGG | 60 | 384 | Paton and Paton 1998 |
hlyA | GCATCATCAAGCGTACGTTCC AATGAGCCAAGCTGGTTAAGCT | 60 | 534 | |
stx1 | TGTAACTGGAAAGGTGGAGTATAC GCTATTCTGAGTCAACGAAAAATAA | 64 | 210 | Sarimehmetoglu et al. 2009 |
stx2 | ATGAAGTGTATATTTAAATGG TCAGTCATTATTAAACTGCAC | 49 | 1200 | Bai et al. 2010 |