Fig. 4

Neighbor-joining phylogenetic tree based on partial 16S rRNA gene sequences from clone libraries of field-grown maize (AG 2040 hybrid) inoculated with A. brasilense Ab-V5 under different N-fertilization conditions. Representative sequences of each OTU not assigned to the Sphingomonadaceae were used to infer the evolutionary history with distances computed by Maximum Composite Likelihood method and presented as the number of base substitutions per site. Numbers at branch points Percentages of 1,000 bootstrap values above a 50 % threshold. The tree was compressed at 1 % base substitution per site. Colored squares Clone library to which each OTU belongs, number in squares number of sequences of each OTU in its respective library