Response of cellular fatty acids to environmental stresses in endophytic Micrococcus spp.

Annals of Microbiology

Table 3 Cellular fatty acids composition of M. aloeverae MCC 2184^T and M. yunnanensis DSM 21948^T in variable conditions of pH

FAME	Micrococcus aloeveare MCC 2184^T				Micrococcus yunnanensis DSM 21948^T
pH	5	7	10	PV	5	7	10	PV
Saturated
12:0	0.22 ± 0.02	0.17 ± 0 .01	nd	0.00	nd	nd	nd	*
14:0	2.37 ± 0.11	1.59 ± 0.27	2.13 ± 0.86	0.25	0.48 ± 0.02	0.52 ± 0.08	0.4 ± 0.05	0.15
16:0	18.64 ± 0.83	8.74 ± 0.37	6.9 ± 2.08	0.00	1.27 ± 0.04	1.16 ± 0.11	1.21 ± 0.10	0.41
17:0	0.33 ± 0.02	2.01 ± 0.66	0.84 ± 0.03	0.00	nd	nd	nd	*
Iso-branched chain
13:0 iso	0.13 ± 0.01	0.19 ± 0.01	nd	0.00	0.27 ± 0.03	nd	0.3 ± 0.02	0.00
14:0 iso	1.63 ± 0.08	1.85 ± 0.33	2.03 ± 0.40	0.35	1.86 ± 0.02	2.07 ± 0.15	2.67 ± 0.17	0.00
15:0 iso	16.2 ± 0.31	15.3 ± 1.96	16.43 ± 1.85	0.69	10.36 ± 0.13	10.04 ± 0.91	14.81 ± 4.87	0.29
16:0 iso	8.32 ± 0.26	7.39 ± 0.60	6.91 ± 0.36	0.02	11.18 ± 0.40	11.51 ± 0.42	13.42 ± 0.36	0.00
17:0 iso	1.05 ± 0.06	0.89 ± 0.05	0.82 ± 0.07	0.01	0.57 ± 0.02	0.52 ± 0.02	0.64 ± 0.03	0.00
Anteiso-branched chain
13:0 ante	0.13 ± 0.05	0.23 ± 0.01	0.24 ± 0.03	0.00	0.36 ± 0.03	0.41 ± 0.01	0.41 ± 0.02	0.08
15:0 ante	44.55 ± 0.60	54.08 ± 1.54	55.9 ± 2.75	0.00	62.16 ± 0.07	63.1 ± 0.48	62.3 ± 3.96	0.87
17:0 ante	6.37 ± 0.26	7.68 ± 1.38	7.63 ± 2.11	0.29	10.78 ± 0.46	10.2 ± 0.62	10.09 ± 1.44	0.50
Unsaturated
16:1 iso H	nd	nd	nd	*	0.35 ± 0.11	0.28 ± 0.01	0.31 ± 0.05	0.58
17:1 ante w9c	nd	nd	nd	*	0.62 ± 0.02	0.32 ± 0.05	0.35 ± 0.01	0.00

Data are means of three biological replicates and are mole % of total fatty acids contents; values after (±) are standard deviations
In most of the cases values of standard errors are almost half the values of standard deviations (data not shown)
Values in bold represent quantitatively dominant fatty acids
nd not detected; PV p value of ANOVA; * p values are not calculated due to single mean
pH 7.0 was treated as a control for comparison of the effects of alkalinity and acidity

ISSN: 1869-2044