Table 1 Production of amyloglucosidase and fungal biomass yield in the presence of various macronutrients and micronutrients
Nutrient components | Specific activity (kU mg−1) | Dry cell mass (mg ml−1) | Nutrient components | Specific activity (kU mg−1) | Dry cell mass (mg ml−1) |
|---|---|---|---|---|---|
Carbon sourceb | Yeast Extracta | ||||
Starch (rice) | 229.5 ± 6.45 | 13.8 ± 0.07 | 0.0 | 164.6 ± 2.45 | 4.90 ± 0.03 |
Starch (commercial) | 208.6 ± 4.89 | 10.6 ± 0.05 | 5.0 | 241.3 ± 7.01 | 17.5 ± 0.08 |
Starch (potato) | 274.0 ± 7.32 | 28.2 ± 1.19 | 10.0 | 274.0 ± 7.32 | 28.2 ± 1.19 |
Starch (sago) | 206.8 ± 4.21 | 9.50 ± 0.05 | 15.0 | 210.0 ± 4.54 | 24.7 ± 0.09 |
Maltose | 93.22 ± 3.98 | 7.90 ± 0.03 | 20.0 | 186.8 ± 3.11 | 23.6 ± 0.09 |
Starch (wheat) | 200.0 ± 4.01 | 11.0 ± 0.05 | Peptonea | ||
Sucrose | 35.06 ± 2.52 | 6.50 ± 0.03 | 0.0 | 171.5 ± 3.21 | 9.70 ± 0.05 |
Wheat bran | 15.45 ± 1.23 | 14.0 ± 0.07 | 5.0 | 274.0 ± 7.32 | 28.2 ± 1.19 |
Bagasse | 5.810 ± 0.05 | 9.50 ± 0.05 | 10.0 | 259.0 ± 6.75 | 16.6 ± 0.08 |
Rice bran | 25.98 ± 1.91 | 10.5 ± 0.05 | 15.0 | 210.3 ± 4.23 | 19.7 ± 0.09 |
Barley | 75.61 ± 3.23 | 8.70 ± 0.04 | 20.0 | 191.6 ± 3.75 | 20.0 ± 0.09 |
Potato starcha | Trace elementsc | ||||
0.0 | 7.430 ± 0.64 | 3.00 ± 0.01 | MgSO4 | 274.0 ± 7.32 | 28.2 ± 1.19 |
5.0 | 205.2 ± 4.14 | 13.0 ± 0.07 | K2HPO4 | 274.0 ± 7.32 | 28.2 ± 1.19 |
10.0 | 274.0 ± 7.32 | 28.2 ± 1.19 | NaCl | 122.8 ± 3.23 | 15.4 ± 0.08 |
15.0 | 233.5 ± 6.98 | 10.8 ± 0.05 | MnSO4 | 23.82 ± 0.98 | 8.30 ± 0.05 |
20.0 | 202.3 ± 4.22 | 8.6 ± 0.04 | CaCl2 | 30.53 ± 1.02 | 15.0 ± 0.09 |
25.0 | 98.57 ± 3.98 | 7.4 ± 0.04 | Magnesium sulphatea | ||
Nitrogen sourceb | 0.5 | 148.7 ± 4.23 | 13.2 ± 0.07 | ||
Peptone | 259.0 ± 6.75 | 16.6 ± 0.08 | 1.0 | 274.0 ± 7.32 | 28.2 ± 1.19 |
Tryptone | 109.0 ± 3.23 | 11.0 ± 0.07 | 1.5 | 132.5 ± 3.78 | 10.2 ± 0.05 |
Ammonium nitrate | 18.60 ± 1.11 | 5.00 ± 0.03 | 2.0 | 102.6 ± 1.11 | 7.50 ± 0.04 |
Potassium nitrate | Nil | 3.80 ± 0.02 | Dipotassium hydrogen phosphatea | ||
Yeast extract | 274.0 ± 7.32 | 28.2 ± 1.19 | 0.5 | 225.7 ± 4.54 | 19.7 ± 0.09 |
Sodium nitrate | Nil | 4.20 ± 0.03 | 1.0 | 274.0 ± 7.32 | 28.2 ± 1.19 |
Urea | 64.27 ± 2.45 | 5.50 ± 0.04 | 1.5 | 232.1 ± 5.87 | 21.0 ± 0.09 |
2.0 | 185.1 ± 3.23 | 18.7 ± 0.09 | |||