Fig. 1
From: Metabolic engineering of Saccharomyces cerevisiae for accumulating pyruvic acid
Schematic representation of the DNA fragments for gene disruption and the disruption of PDC1 by homologous recombinant. a Structure of DNA fragments I–III. Fragment I denotes PCR product of S. cerevisiae PDC1; Fragment II denotes PCR product of Kan r; Fragment III denotes PCR product containing S. cerevisiae PDC1-F1, Kan r, and S. cerevisiae PDC1-F2 (from left to right); b PDC1 gene disruption by homologous recombination