Fig. 2
From: Metabolic engineering of Saccharomyces cerevisiae for accumulating pyruvic acid
Schematic representation of DNA fragments for gene disruption and disruption of PDC5 by homologous recombination. a Structure of DNA fragments I–III. Fragment I denotes the PCR product of S. cerevisiae PDC5; Fragment II denotes the PCR product of His4; Fragment III denotes the PCR product containing S. cerevisiae PDC5-F1, His4, and S. cerevisiae PDC5-F2 (from left to right). b PDC5 gene disruption by homologous recombination