Fig. 3
From: Metabolic engineering of Saccharomyces cerevisiae for accumulating pyruvic acid
Construction of the deletion cassette and verification of the strains Y2-1 (ΔPDC1) and Y2-15 (ΔPDC1 ΔPDC5). a Analysis of TP1K digested by EcoRI and BglII. M: DNA Marker III; 1: TP1K digested by EcoRI and BglII; 2: TP1K. b Analysis of TP1K digested by NotI. M: 1-kb DNA Marker; 1: TP1K digested by NotI; 2: TP1K. c Electrophoresis analysis of PCR products of Y2-1. M: DNA Marker III; 1, 2, and 3 are the PCR products of TP1K, Y2-1, and Y2, respectively. d Electrophoresis analysis of PCR products of Kan r. M: 1 kb DNA Marker; 1, 2, and 3 are the PCR products of TP1K, Y2-1, and Y2, respectively. e Analysis of TP5H digested by EcoRI and HindIII. M: 1 kb DNA Marker; 1:TP5H digested by EcoRI and HindIII; 2: TP5H. f Analysis of TP5H digested by NotI. M: 1 kb DNA Marker; 1: TP5H digested by NotI; 2: TP5H. g Electrophoresis analysis of PCR products of Y2-15. M: 1 kb DNA Marker; 1, 2, and 3 are the PCR products of TP5H, Y2-15, and Y2, respectively. h Electrophoresis analysis of PCR products of His4. M: 1 kb DNA Marker; 1, 2, and 3 are the PCR products of TP5H,Y2-15 and Y2, respectively