Fig. 5From: Metabolic engineering of Saccharomyces cerevisiae for accumulating pyruvic acidGrowth curves of the parent strain Y2 and the mutants Y2-1 and Y2-15. Strains were pre-grown for 2 days in fresh YNBG medium to stationary phase, and 5 mL cultures were placed into 500 mL flasks containing 50 mL of fresh YNBG medium and shaken at 200 rpm for 24 h at 30 °CBack to article page