Fig. 6From: Metabolic engineering of Saccharomyces cerevisiae for accumulating pyruvic acidDetermination of PDC activity of S. cerevisiae. Strains were pre-grown for 2 days in fresh YNBG medium to stationary phase, and 5-mL cultures were placed into 500 mL flasks containing 50 mL of fresh fermentation medium and shaken at 200 rpm for 12 h at 30 °CBack to article page