Table 1 Strains and plasmids used in the study
From: Metabolic engineering of Saccharomyces cerevisiae for accumulating pyruvic acid
Strains or plasmids | Related characteristics | Source |
|---|---|---|
Strain | ||
E. coli DH5α | supE44△lacU169(ψ80lacZ△M15) hsdR17recA1endA1gyrA96thi-1relA1 | Tianjin University of Science and Technology |
S. cerevisiae Y2 | his4 | Tianjin University of Science and Technology |
S. cerevisiae Y2-1 | his4 PDC1::kan r | This work |
S. cerevisiae Y2-15 | his4 PDC1::kan r PDC5::his4 | This work |
Plasmid | ||
pGEM-T | Nankai University | |
pPIC-3.5 K | Nankai University | |
pMD19-T | TAKARA | |
PT-1 | This work | |
T-K | This work | |
TP1K | This work | |
PT-5 | This work | |
T-H | This work | |
TP5H | This work | |