Table 1 Primers used in this study
Target gene | Primer sequences 5′-3′ | Annealing temperature Tm (°C) | PCR product (bp) |
|---|---|---|---|
aph(2″)Ib | F- GCAAATGGCACAGTATAATATGC | 56 | 958 |
R- GCTTGTGTTTGTAGCAATTCAG | |||
aph(3′)IIIa | F- GATACGGAAGGAATGTCTCC | 56 | 663 |
R- GCTTGATCCCCAGTAAGTC | |||
aad6/ ant6 | F - CTTTAGCAGAACAGGATG | 52 | 844 |
R- CCGGCCTCTCTTCTATATC | |||
ant(6)Ia | F- CGCCTCTCTTCTATATCA | 50 | 844 |
R- CTTTAGCAGAACAGGATG | |||
ant(9)Ia | F-CCAAATCAAGCGATTCAAAC | 54 | 690 |
R- TCCTTCCCACTTATCATCC | |||
ant(9)Ib | F- GATCAGGAGTTGAGAGTG | 46 | 543 |
R- GAGAAGATTCAGCCACTG |