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Fig. 5 | Annals of Microbiology

Fig. 5

From: Protoplasting, regeneration and transformation of medicinal mushroom Ganoderma multipileum using succinate dehydrogenase mutation gene as a selection marker

Fig. 5

a,b Electrophoresis of PCR-amplified Sdh subunit B gene fragments from Sdh gene point mutated transformants of G. multipileum BCRC 37180 without (a) or with (b) AgeI restriction. a Multiple Sdh gene fragments amplified using gmSdhB-Sense 1490–0503 and gmSdhB-AS-0503 primers. The anticipated Sdh gene fragment length of Sdh subunit B gene is 1900 bp (arrow). b The 1900 bp amplicon restriction fragment length polymorphism (RFLP) of wild type (WT) and transformants (T1, T2, T3, T4) restricted by AgeI. Bands at 600 bp and 1300 bp are AgeI restriction products of transformed carboxin resistance cassette (arrowheads). T4 is a false positive transformant. M 1 kb marker, length size at 2000, 1500 and 1000 bp denoted (arrows)

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Annals of Microbiology

ISSN: 1869-2044

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