Table 1 Identification of yeasts isolated from grape must collected from four wineries (Barletta, San Severo, Lucera, Ascoli Satriano) by internal transcribed spacer–restriction fragment length polymorphism (ITS-RFLP) analysis and sequencing
Species a | ITS | Restriction fragments | Number of isolates | Origin | Accession number | ||||
|---|---|---|---|---|---|---|---|---|---|
HaeIII | HinfI | CfoI | DdeI | 2012 | 2013 | ||||
Candida boidinii | 750 | 700 | 390 + 190 + 160 | 350 + 310 + 90 | / | 12 | 8 | Barletta | KP241860-KP241861 |
Candida zemplinina | 475 | 475 | 235 + 235 | 215 + 110 + 80 + 60 | / | 9 | 12 | Barletta | KP241864-KP241865 |
Hanseniaspora guilliermondii | 750 | 750 | 350 + 200 + 180 | 320 + 310 + 105 | 380 + 180 + 95 + 80 | 25 | 30 | Barletta | KP241874-KP241875 |
Issatchenkia terricola | 450 | 290 + 125 | 240 + 105 + 105 | 130 + 100 + 90 + 85 + 45 | / | 2 | / | Barletta | KP241882-KP241883 |
Zygosaccharomyces bailii | 790 | 690 + 90 | 340 + 225 + 160 + 55 | 320 + 270 + 95 + 95 | / | 2 | / | Barletta | KP241898-KP241899 |
Candida boidinii | 750 | 700 | 390 + 190 + 160 | 350 + 310 + 90 | / | 7 | 2 | San Severo | KP2281424-KP281425 |
Candida zemplinina | 475 | 475 | 235 + 235 | 215 + 110 + 80 + 60 | / | 12 | 10 | San Severo | KP241866-KP241867 |
Hanseniaspora guilliermondii | 750 | 750 | 350 + 200 + 180 | 320 + 310 + 105 | 380 + 180 + 95 + 80 | 22 | 24 | San Severo | KP241876-KP241877 |
Issatchenkia terricola | 450 | 290 + 125 | 240 + 105 + 105 | 130 + 100 + 90 + 85 + 45 | / | 6 | 5 | San Severo | KP241884-KP241885 |
Hanseniaspora uvarum | 750 | 750 | 350 + 200 + 180 | 320 + 310 + 105 | 300 + 180 + 95 + 90 + 85 | 1 | 8 | San Severo | KP241872-KP241873 |
Zygoascus hellenicus | 650 | 625 | 350 + 170 + 130 | 325 + 325 | / | 2 | 1 | San Severo | KP241902-KP241903 |
Candida boidinii | 750 | 700 | 390 + 190 + 160 | 350 + 310 + 90 | / | 10 | 4 | Lucera | KP2281426-KP281427 |
Candida zemplinina | 475 | 475 | 235 + 235 | 215 + 110 + 80 + 60 | / | 8 | 10 | Lucera | KP2281428-KP281429 |
Hanseniaspora guilliermondii | 750 | 750 | 350 + 200 + 180 | 320 + 310 + 105 | 380 + 180 + 95 + 80 | 26 | 26 | Lucera | KP241878-KP241879 |
Issatchenkia terricola | 450 | 290 + 125 | 240 + 105 + 105 | 130 + 100 + 90 + 85 + 45 | / | 2 | 1 | Lucera | KP241886-KP241887 |
Zygosaccharomyces bailii | 790 | 690 + 90 | 340 + 225 + 160 + 55 | 320 + 270 + 95 + 95 | / | 2 | 1 | Lucera | KP241900-KP241901 |
Hanseniaspora uvarum | 750 | 750 | 350 + 200 + 180 | 320 + 310 + 105 | 300 + 180 + 95 + 90 + 85 | 1 | 6 | Lucera | KP241870-KP241871 |
Hanseniaspora opuntiae | 750 | 750 | 350 + 200 + 180 | 320 + 310 + 105 | / | 1 | 2 | Lucera | KP241868-KP241869 |
Candida boidinii | 750 | 700 | 390 + 190 + 160 | 350 + 310 + 90 | / | 12 | 8 | Ascoli Satriano | KP241862-KP241863 |
Candida zemplinina | 475 | 475 | 235 + 235 | 215 + 110 + 80 + 60 | / | 6 | 10 | Ascoli Satriano | KP2281430-KP281431 |
Hanseniaspora guilliermondii | 750 | 750 | 350 + 200 + 180 | 320 + 310 + 105 | 380 + 180 + 95 + 80 | 30 | 30 | Ascoli Satriano | KP241880-KP241881 |
Issatchenkia terricola | 450 | 290 + 125 | 240 + 105 + 105 | 130 + 100 + 90 + 85 + 45 | / | 2 | 2 | Ascoli Satriano | KP241888-KP241889 |