The gut microbiota of the wood-feeding termite Reticulitermes lucifugus (Isoptera; Rhinotermitidae)

Annals of Microbiology

Table 2 Results of isolation of cellulolytic gut bacteria from enrichment cultures on different media using various lignocellulose substrates as C source

Enrichment	Number of isolates on isolation media				Cellulolytic isolates^b
Enrichment	W2		W3		Cellulolytic isolates^b
C source	CMC^a	Xyl	CMC	Xyl	%
CMC	23	6	5	1	37.1
Walnut wood	10	34	0	22	6.1
Eucalyptus wood	20	23	20	17	10.0
Filter paper	16	15	16	14	35.0
Pine wood	10	21	12	11	22.2
All	79	99	53	65	19.7

After 1 month of incubation, the cultures were inoculated on W2 and W3 solid media containing CMC and xylan, respectively
CMC carboxymethylcellulose
^aThe composition of W2 andW3 media is reported in the "Materials and Methods" section
^bPositive isolates to the Congo red test

ISSN: 1869-2044