Table 1 Strains, plasmids, and primers used in this study
Name | Characteristics | Source |
|---|---|---|
Strains | ||
TRTH | trpEDCBA + TetR, Δtna | Liu et al. 2012 |
TRTHA | Derived from TRTH, but ΔackA | This study |
TRTHT | Derived from TRTH, but ΔtdcD | This study |
TRTHAT | Derived from TRTH, but ΔackA, ΔtdcD | This study |
Plasmids | ||
pKD46 | AmR, λ Red-expressing vector | Cherepanov and Wackernagel 1995 |
pKD3 | CmR, Template vector | Cherepanov and Wackernagel 1995 |
pCP20 | AmR, CmR, FLP-expressing vector | Cherepanov and Wackernagel 1995 |
Primers | ||
ackA-P1 | 5’-CTGTCCCCGGCGAAACAAGCTAAAAAAATTAACAGAACGATTATCCGGCGTTGACATTGAGCGATTGTGT AGGCTGGAG-3’b | This study |
ackA-P2 | 5’-CGGATCACGCCAAGGCTGACGCTGGTCAGACCGACGCTGGTTCCGGTAGGGATCAGTAACGGCTGACATGGGAATTAGC-3’b | This study |
ackA-P3 | 5’-TGCCCAGCCACCACAATC-3’ | This study |
ackA-P4 | 5’-GTGGTAGTTTGCGACGAT-3’ | This study |
tdcD-P1 | 5’-GTGGGAGAGATCTCACTAAAAACTGGGGATACGCCTTAAATGGCGAAGAAACGGTTTGAGCGATTGTGTAGGCTGGAG-3’b | This study |
tdcD-P2 | 5’-CATCCTGAACATCGTATACAAACTGTTTTAATCCGTAACTCAGGATGAGAAAAGAGTAACGGCTGACATGGGAATTAGC-3’b | This study |
tdcD-P3 | 5’-CGGGCGGACCAAATGATAC-3’ | This study |
tdcD-P4 | 5’-AACCCGAACATCCTTGAC-3’ | This study |