Table 2 Effect of medium components on cell growth and subtilosin production
AF-MRS modification | Zone of growth inhibition (mm) | Standard deviation (σ) | Bacteriocin activity (AU mL−1) | Maximal OD600 (1:10 dilution) | Initial pH | Final pH |
|---|---|---|---|---|---|---|
Tween 80 | ||||||
0 | 15 | 1.2 | 160 | 0.75 | 6.1 | 5.9 |
0.025 %a | 15 | 0.7 | 160 | 0.67 | 6.1 | 5.9 |
0.050 % | 16 | 0.5 | 267 | 0.65 | 6.1 | 5.9 |
0.100 % | 17 | 1.7 | 213 | 0.67 | 6.1 | 6.0 |
0.200 % | 16 | 1.3 | 267 | 0.58 | 6.0 | 6.0 |
Control (MRS)b | 18 | 1.8 | 320 | 0.68 | 6.4 | 6.2 |
Carbohydrates | ||||||
None | 18 | 0.3 | 240 | 0.47 | 6.4 | 7.7 |
2.0 % dextrose | 15 | 0.3 | 80 | 0.58 | 6.2 | 5.9 |
2.0 % sucrose | 13 | 0.5 | 40 | 0.62 | 6.4 | 5.8 |
2.0 % corn starch | 18 | 0 | 160 | 0.41 | 6.4 | 6.7 |
2.0 % maltodextrin | 18 | 0.4 | 240 | 0.56 | 6.4 | 6.7 |
2.0 % amylopectin | 18 | 0.3 | 240 | 0.41 | 6.4 | 6.7 |
Control (MRS) | 16 | 0.3 | 107 | 0.66 | 6.3 | 6.0 |
Nitrogen | ||||||
2.5 % Freetone A-1 | 17 | 1.7 | 133 | 0.54 | 6.3 | 5.8 |
2.5 % Martone L-1 | 18 | 1.4 | 213 | 0.60 | 6.2 | 5.9 |
2.5 % Cottonseed Hydrolysate | 18 | 1.3 | 213 | 0.65 | 6.4 | 6.1 |
2.5 % Cottonseed Hydrolysate-UF | 18 | 1.4 | 213 | 0.76 | 6.3 | 6.0 |
1.25 % Freetone A-1 plus 1.25 % Martone L-1 | 17 | 1.6 | 133 | 0.59 | 6.3 | 5.8 |
Control (MRS) | 19 | 1.1 | 267 | 0.63 | 6.3 | 6.0 |
0 % yeast extract (YE) | 16 | 1.4 | 144 | 0.58 | 6.2 | 5.8 |
0.25 % YE (bac grade)c | 17 | 1.3 | 160 | 0.64 | 6.2 | 6.1 |
0.50 % YE (bac grade) | 16 | 1.1 | 176 | 0.58 | 6.2 | 6.0 |
0.75 % YE (bac grade) | 17 | 1.6 | 213 | 0.58 | 6.2 | 6.1 |
0.50 % YE (processed)d | 16 | 0.5 | 120 | 0.49 | 6.2 | 5.6 |
0.25 % YE (tech grade)e | 17 | 0.9 | 224 | 0.65 | 6.2 | 6.0 |
0.50 % YE (tech grade) | 17 | 1.1 | 192 | 0.61 | 6.2 | 5.8 |
0.75 % YE (tech grade) | 17 | 1.2 | 200 | 0.61 | 6.2 | 6.0 |
Control (MRS) | 17 | 0.6 | 160 | 0.53 | 6.2 | 5.9 |
0 % ammonium citrate | 17 | 1.5 | 213 | 0.73 | 6.2 | 5.9 |
0.01 % ammonium citrate | 17 | 2.6 | 187 | 0.71 | 6.0 | 5.9 |
0.02 % ammonium citrate | 18 | 2.4 | 160 | 0.83 | 5.8 | 5.9 |
0.04 % ammonium citrate | 14 | 2.4 | 45 | 0.65 | 5.6 | 5.6 |
Control (MRS) | 20 | 1.7 | 205 | 0.46 | 6.3 | 5.6 |
Sodium Acetate | ||||||
0 % | 18 | 1.1 | 373 | 0.72 | 5.9 | 6.3 |
0.01 % | 18 | 1.2 | 213 | 0.62 | 5.9 | 6.0 |
0.02 % | 17 | 1.2 | 133 | 0.49 | 5.9 | 5.7 |
0.04 % | 14 | 1.5 | 93 | 0.41 | 6.0 | 5.8 |
Control (MRS) | 16 | 0.7 | 160 | 0.55 | 6.3 | 6.0 |
MgSO4 | ||||||
0 % | 16 | 1.7 | 160 | 0.47 | 6.1 | 5.7 |
0.0025 % | 16 | 1.6 | 133 | 0.51 | 6.1 | 5.6 |
0.005 % | 16 | 1.6 | 133 | 0.50 | 6.1 | 5.6 |
0.010 % | 16 | 1.3 | 120 | 0.47 | 6.1 | 5.7 |
0.020 % | 16 | 1.3 | 213 | 0.51 | 6.1 | 5.6 |
Control (MRS) | 16 | 0.7 | 160 | 0.55 | 6.2 | 6.0 |
MnSO4 | ||||||
0 % | 18 | 1.6 | 240 | 0.58 | 6.1 | 6.1 |
0.0010 % | 16 | 2.4 | 240 | 0.58 | 6.1 | 6.1 |
0.0025 % | 17 | 2.1 | 187 | 0.61 | 6.1 | 6.1 |
0.0050 % | 17 | 2.3 | 160 | 0.57 | 6.1 | 6.0 |
0.0100 % | 17 | 2.7 | 173 | 0.57 | 6.1 | 6.1 |
Control (MRS) | 17 | 0.5 | 160 | 0.58 | 6.2 | 5.8 |
K2HPO4 | ||||||
0 % | 0 | 0 | 0 | 0.16 | 5.6 | 5.9 |
0.1 % | 18 | 0.7 | 140 | 0.50 | 5.8 | 5.8 |
0.2 % | 18 | 1.6 | 151 | 0.63 | 6.1 | 5.7 |
0.4 % | 19 | 1.4 | 191 | 0.70 | 6.3 | 5.8 |
0.8 % | 18 | 1.2 | 160 | 0.59 | 6.6 | 6.0 |
1.6 % | 19 | 0.5 | 160 | 0.55 | 6.9 | 6.6 |
2.0 % | 18 | 1.0 | 160 | 0.50 | 6.9 | 6.7 |
Control (MRS) | 19 | 1.9 | 255 | 0.68 | 6.3 | 5.8 |