Table 3 Effect of culture volume and agitation on cell growth and subtilosin production in AF-MRS
Volume (mL) | Agitation (rpm) | Zone of growth inhibition (mm) | Standard deviation (σ) | Bacteriocin activity (AU mL−1) | Maximal OD600 (1:10 dilution) | Initial pH | Final pH |
|---|---|---|---|---|---|---|---|
25 | 0 | 11 | 3.1 | 87 | 0.40 | 6.3 | 6.0 |
25 | 140 | 16 | 0.6 | 133 | 0.61 | 6.3 | 5.9 |
25 | 220 | 18 | 0.5 | 267 | 0.91 | 6.3 | 7.0 |
25 | 300 | 17 | 1.1 | 320 | 1.02 | 6.3 | 7.2 |
50 | 0 | 11 | 3.5 | 73 | 0.22 | 6.3 | 6.0 |
50 | 140 | 14 | 0.8 | 187 | 0.28 | 6.3 | 5.6 |
50 | 220 | 16 | 1.5 | 187 | 0.51 | 6.3 | 6.0 |
50 | 300 | 16 | 0.9 | 187 | 0.66 | 6.3 | 6.7 |
100 | 0 | 8 | 1.3 | 30 | 0.09 | 6.3 | 6.0 |
100 | 140 | 13 | 0.6 | 60 | 0.25 | 6.3 | 5.6 |
100 | 220 | 14 | 0.7 | 133 | 0.24 | 6.3 | 5.5 |
100 | 300 | 15 | 0.7 | 133 | 0.47 | 6.3 | 5.6 |
50a | 220 | 17 | 1.1 | 267 | 0.54 | 6.6 | 6.3 |