Subtilosin A production by Bacillus subtilis KATMIRA1933 and colony morphology are influenced by the growth medium

Table 4 Cell growth and subtilosin production by morphological variants VF and VP of strain KATMIRA1933 in AF-MRS and unmodified MRS

Variant and growth medium	Zone of growth inhibition (mm)	Standard deviation (σ)	Bacteriocin Activity (AU mL⁻¹)	Maximal OD₆₀₀ (1:10 dilution)	Initial pH	Final pH
VF, AF-MRS	17	0.5	213	0.48	6.1	5.7
VP, AF-MRS	17	1.9	213	0.52	6.1	5.9
VM^a, AF-MRS	15	0.7	150	0.48	6.1	5.6
VF, MRS (unmodified)	18	0.7	304	0.59	6.1	5.7
VP, MRS (unmodified)	18	2.0	272	0.56	6.1	5.8
VM, MRS (unmodified)	16	1.1	200	0.48	6.2	5.6

VM^a = combination of VF and VP
All cultures were incubated at 37 °C in 250 mL glass flasks on an orbital shaker (220 rpm) for 15 h. Each flask contained 50 mL medium. Values listed are the averages of three experiments

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