Subtilosin A production by Bacillus subtilis KATMIRA1933 and colony morphology are influenced by the growth medium

Table 5 Subtilosin activity in MRS, AF-MRS, and AF-MRS_OPT recorded at different time points

Culture medium	Harvest time (h)	Standard deviation (σ)	Bacteriocin activity (AU mL⁻¹)	Zone of inhibition (mm)
MRS	15	0	None	None
MRS	26	0.4	40	10
MRS	46	0.4	40	11
MRS	60	0.4	80	13
AF-MRS	26	0.5	20	10
AF-MRS_OPT	15	0.7	80	12
AF-MRS_OPT	26	0.5	80	13

All cultures were incubated at 37 °C in 3-L glass flasks, placed on magnetic stirrers set at maximum speed. Each flask contained 2 L medium. Values listed are the averages of three experiments

ISSN: 1869-2044