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Table 6 Klykov’s model (2011) parameters for flask incubation at different agitation conditions where “X” is measured in O.D. units

From: Subtilosin A production by Bacillus subtilis KATMIRA1933 and colony morphology are influenced by the growth medium

A

XLim

Xp

μmax

X l

XLim st

nLim

PLim

kdiv

kst

0.0125

0.6

1.09

0.0041

0.33

0.30

140

140

5.38

0

  1. LGP is a logarithmic growth phase; GIP is a growth inhibition phase; S substrate concentration, g l−1; X is a biomass concentration, OD units or g L−1; OD is a unit of an optical density; τ is time, hour; P is products-metalloproteinase, units P mL−1; A = m/a (1) is a parameter that describes a delay of the biomass growth rate; (2) specific rate of accumulation of stable cells, h−1; X p is maximum biomass concentration, when all the energy generated during cultivation is consumed for cell viability maintenance; X Lim is biomass concentration in the end of exponential growth phase and beginning of growth inhibition phase; X st is a concentration of the biomass of zero age cells (stable), the content of resting cells, OD units or g L−1; X div= X − X st is a concentration of proliferation biomass, OD units or g L−1; τ Lim is a time of exponential growth phase termination, hour; X Lim st is a concentration of stable cells at the end of exponential growth phase, OD units or g L−1; nLim is an agitation at the end of exponential growth phase; R is a ratio of X st to biomass X, relative content of stable cells in the biomass, synchronization degree, part of 1; k p div , k p st , k s div , k s st are constants of metabolite and substrate biochemical reaction rates, units of P (or S)/(mL units of biomass X per one hour); X l is an initial biomass concentration in LGP corresponding to the beginning of population structuring, OD units or g L−1; P Lim is metabolite concentration or activity at the end of LGP and beginning of GIP, units of P mL−1