Fig. 1

Structure of pFungiway vectors. The structure of the DNA construct inside the T-DNA region of each pFungiway vector is indicated. pFungiway1, 2, 3, 4, and 7 are expression vectors, while pFungiway5, 6, and 8 are repression vectors. The black boxes indicate the first intron of the glyceraldehyde 3-phosphate dehydrogenase gene (gpd) derived from Flammulina velutipes. The boxes with diagonal lines indicate the truncated egfp. The arrows indicate the direction of transcription and the letters ATG denote the transcription initiation codon. The apparent size of each box is not proportional to the actual length of each component. Abbreviations: LB left border of T-DNA, RB right border of T-DNA, Pgpd promoter of the gpd gene of Flammulina velutipes¸ GateA and GateB reading frame cassette A and B provided by the Gateway technology, TtrpC terminator of the trpC gene of Aspergillus nidulans, T35S terminator of the cauliflower mosaic virus 35G gene, hph hygromycin B phosphotransferase, nptII neomycin phosphotransferase