Fig. 4
From: pFungiway: a series of plasmid vectors used for gene manipulation in fungi
Effect of RNAi on the amount of cob transcript in cells of Fusarium oxysporum transformants. The amount of transcript was evaluated by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), using the β-tubulin gene as an internal control for quantitative standardization. Lanes: 1–3 independent transformant strains possessing both full-length cob (by pFungiway2-cob) and the RNAi construct of partial cob (by pFungiway6-cob); U transformant strain with pFungiway2-cob alone; C parental strain (control)