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Table 1 Oligonucleotides used for polymerase chain reaction (PCR) amplification

From: pFungiway: a series of plasmid vectors used for gene manipulation in fungi

Oligonucleotide

Sequence (5′-3′)

EGFPfw

caccATGGTGAGCAAGGGCGAGGA

EGFPrv

TTACTTGTACAGCTCGTCCATGCC

DsRedfw

caccATGGCCTCCTCCGA

DsRedrv

CCGCTACAGGAACAGGTG

UMTfw

caccATGCATGACATCGACTATCG

UMTrv

GAAATACCTGCCGAGGCG

UMT-Intfw

caccATGACATCGACTAT

UMT-Intrv

TTGTCACTGCCGAACCGT

Tubfw

AAATCGGTGCTGCTTTCTGG

Tubrv

ACGACGGTGTCAGAGACCTT

  1. Lowercase letters denote an additional sequence required for a directional cloning toward the pENTR/D-TOPO vector