Fig. 7From: Degenerate primers as biomarker for gene-targeted metagenomics of the catechol 1, 2-dioxygenase-encoding gene in microbial populations of petroleum-contaminated environmentsMolecular analysis of GSN28 isolate. a 360-bp fragment was produced by Touchdown-gradient PCR using the INDO-U-F and INDO-U-R primer pair in the GSN28 isolate. b The specific cDNA amplified by reverse transcription-PCR by the INDO-U-R and Oligo dG primers. Lane M 100-bp DNA markerBack to article page