Fig. 8From: Degenerate primers as biomarker for gene-targeted metagenomics of the catechol 1, 2-dioxygenase-encoding gene in microbial populations of petroleum-contaminated environmentsTotal environmental cDNA amplification: a The 500-bp environmental cDNA of catechol 1, 2-dioxygenase was amplified by reverse transcription-PCR. b Specific environmental cDNA was extracted from the agarose gel. Lane M 100Â bp DNA markerBack to article page