Degenerate primers as biomarker for gene-targeted metagenomics of the catechol 1, 2-dioxygenase-encoding gene in microbial populations of petroleum-contaminated environments

Table 1 Final pH value and absorbance of culture broth for selected and identified isolates after 72 h of culture in broth supplemented with two different concentrations of phenol

Selected strain	The closest species according to 16S rDNA sequence similarity	GenBank accession numbers for isolated bacteria	1000 mg Phenol l⁻¹		2000 mg Phenol l⁻¹
Selected strain		GenBank accession numbers for isolated bacteria	Absorbance (600 nm)	pH	Absorbance (600 nm)	pH
GSN3	Acinetobacter calcoaceticus DSM30006	KF815689	1.454	5.49	0.189	5.53
GSN4	Pseudomonas resinovorans CA10	KF815690	1.432	5.56	0.047	6
GSN5	Pseudomonas jinjuensis Pss26	KF815691	0.983	5.77	0.171	5.87
GSN6	Cupriavidus oxalaticus NBRC	KF815692	0.07	6.92	0.065	6.11
GSN8	Acinetobacter calcoaceticus DSM30006	KF815693	0.894	6.04	0.092	5.8
GSN9	Pseudomonas pseudoalcaligenes KF707	KF815694	0.83	6.83	0.05	6
GSN12	Pseudomonas pseudoalcaligenes KF707	KF815694	1.18	5.96	0.072	5.8
GSN11	Pseudomonas putida SP2	KF815695	1.24	6.06	0.15	5.92
GSN13	Pseudomonas jinjuensis Pss26	KF815696	0.954	5.9	0.27	6.01
GSN15	Acinetobacter oleivorans DR1	KF815697	1.538	4.92	0.091	6
GSN16	Pseudomonas knackmussii B13	KF815698	0.81	5.38	0.097	6.07
GSN20	Acinetobacter genomosp. 3 LUH6175	KF815699	0.342	6.54	0.17	5.65
GSN21	Pseudomonas knackmussii B13	KF815700	0.85	5.43	0.103	6
GSN22	Pseudomonas plecoglossicida R8-323	KF815701	0.285	6.3	0.135	5.72
GSN23	Pseudomonas monteilii EU45	KF815702	1.41	5.82	0.185	5.34
GSN26	Pseudomonas aeruginosa L-4	KF815703	0.45	5.34	0.181	5.77
GSN28	Pseudomonas citronellolis BG6903	KF815704	0.36	5.42	0.42	5.25

ISSN: 1869-2044