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Table 2 Identification of peptides from activated proteins of B.t.LDC 501a using mass spectrometric analysis

From: Screening and characterization of a non-insecticidal Bacillus thuringiensis strain producing parasporal protein with selective toxicity against human colon cancer cell lines

Protein identified

Accession

Domain

Sequence

Observed molecular weight (kDa)

Experimental molecular weight (kDa)

pI

Score

Coverage (%)

No. of peptides identified

Uncharacterized protein

EEM47994.1

TNF-like domains

VLFQNEQFDLANEYNPVTSIFTPK

66

21.5 (trimer)

7.97

84.85

40.31

5

Stage 0 sporulation protein A

AJG78136.1

CheY-like superfamily

VcLVDDNKELVSmLESYVAAQDDMEVIGTAYNGQEcLNLLK

30

30.7

8.31

20.90

21.01

3

Uncharacterized protein

AJH08277.1

TNF-like domains and C1q domain

NTVNQNVPANTFVK

20

19.9

7.69

2.72

31.46

4

  1. TNF, Tumour necrosis factor
  2. a B.t.LDC 501 refers to a Bacillus thuringiensis strain with parasporal inclusions which was determined to be both non-haemolytic and non-insecticidal and to show cytotoxicity on all of the human colon cancer cell lines tested