Supplementary Figure S1

Agarose gel (0.8 % W/V in TBE buffer) electrophoresis images. Lane 1 (ML) 6 μL of Gene Ruler DNA 1-kb Ladder (Thermo Fisher, Waltham, MA, USA). Upper labels indicate the name of the plasmid loaded (each lane corresponds to a sample from duplicate experiments). 50 ng of pDNA (measured spectrophotometrically) were loaded on each of these lanes. The gel was stained with Sybr Green (Sigama Aldrich, MO, USA) using 1.5 μL of Sybr Green per 30 mL of gel. The gel was run at 80 V for 30 min. A Samples from cultures in LB medium, B samples from cultures in TB medium, C Samples from EBP medium. (GIF 186 kb)