Serial Number | Tests | Characteristics |
---|---|---|
1. | Colony colour | Milky-white |
2. | Catalase test | Negative |
3. | Gram staining | Positive |
4. | Cell shape | Rod-shaped |
5. | Colony morphology | Small, dome-shaped |
6. | Acid tolerance | Positive |
7. | Acidifying activity | Positive |
8. | Response to antibiotics: | |
a) Streptomycin, gentamicin, kanamycin | Resistance | |
b) Erythromycin, tetracycline, chloramphenicol | Sensitive | |
9. | Ferment carbohydrates mannose, fructose, dextrose, trehalose, sucrose, maltose, cellobiose, esculin and inulin | Positive |
10. | NaCl concentrations (%): | Growth (OD600) |
a) Control (without NaCl) | 1.68 ± 0.032 | |
b) 0.5 | 0.68 ± 0.029 | |
c) 6.5 | Nil | |
d) 10 | Nil | |
e) 15 | Nil | |
f) 20 | Nil |