Table 2 Biochemical properties of bacteriocin LD4

1

Control (untreated)

20.12 ± 0.32

2

Temperatures

 a) 60 °C, 15 min

20.14 ± 0.48

 b) 80 °C, 15 min

20.12 ± 0.64

 c) 100 °C, 15 min

20.19 ± 0.28

 d) 121 °C at 15 psi, 15 min

19.18 ± 0.33

3

pH

 a) pH 2.0

20.08 ± 0.30

 b) pH 4.6 (control)

20.12 ± 0.40

 c) pH 6.0

14.00 ± 0.43

 d) pH 7.0

8.08 ± 0.65

 e) pH 8.0

Nil

4

Organic solvents, surfactants, and detergents

 a) Ethanol

20.08 ± 0.32

 b) Methanol

20.08 ± 0.21

 c) Isopropanol

20.08 ± 0.28

 d) Acetone

20.08 ± 0.29

 e) Ethyl acetate

20.08 ± 0.31

 f) Acetonitrile

20.08 ± 0.40

 g) Urea

20.08 ± 0.37

 h) Tween 80

20.08 ± 0.26

 i) Triton X-100

20.08 ± 0.29

 j) SDS

20.08 ± 0.32

5

Hydrolyzing enzymes

 a) Catalase

20.07 ± 0.21

 b) α-Amylase

20.18 ± 0.20

 c) Lipase

20.08 ± 0.18

 d) Trypsin

16.21 ± 0.29

 b) α-Chymotrypsin

19.12 ± 0.32

 c) Proteinase K

19.68 ± 0.28

 d) Protease

16.38 ± 0.27

 e) Pepsin

20.00 ± 0.28