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Table 1 Gradient elution program for the bioamine assay

From: Hop resistance and beer-spoilage features of foodborne Bacillus cereus newly isolated from filtration-sterilized draft beer

Buffer

Time (min)

0

10

15

20

27

30

35

40

45

Buffer A (pure water) (%)

35

35

25

20

15

10

5

35

35

Buffer B (methanol) (%)

65

65

75

80

85

90

95

65

65

  1. The bioamine assay comprised high-performance liquid chromatography, the mobile phase of which was consisted of pure water (Buffer A) and methanol (Buffer B)